BACTERIOLOGY AND MYCOLOGY QUESTION ANSWERS
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| BACTERIOLOGY AND MYCOLOGY QUESTION ANSWERS |
Part 1:
- Fletcher’s media is used for cultivation of ………………………………
a. Borrelia
b. Leptospira
c. Salmonella
d. Listeria
ANS: b. Leptospira - For motility testing ……………………. Media is used
a. Liquid media
b. Semi solid media
c. Solid media
d. All the above
ANS: b. Semi solid media - Tryptic soy agar is a ……………………… media
a. Basal
b. Enriched
c. Selective
d. Enrichment
ANS: a. Basal - Concentration of salt in Mannitol salt agar is ………………………..
a. 2%
b. 6%
c. 3%
d. 5%
ANS: d. 5% - Egg containing media is used for the cultivation of ……………………………..
a. Mycobacteria
b. Mycoplasma
c. Chlamydia
d. Borrelia
ANS: a. Mycobacteria - Rhodamine will give …………………….. fluorescence
a. Apple green
b. Orange
c. Reddish
d. Bluish
ANS: c. Reddish - Mesosome is the invagination of ………………………..
a. Nuclear membrane
b. Plasma membrane
c. Cell wall
d. Capsule
ANS: b. Plasma membrane - Which among the following is having linear DNA?
a. Bordettela
b. Borrelia
c. Brucella
d. Blue green algae
ANS: b. Borrelia - Volutin granules are seen in ………………………….
a. Corynebacterium
b. Campylobacter
c. Clostridia
d. Listeria
ANS: a. Corynebacterium - …………………………….. is the numerically most abundant protein of gram –ve cell wall
a. Lipoprotein
b. Glycoprotein
c. Apoprotein
d. Flagellim
ANS: a. Lipoprotein - Flagellar antigen is …………………………
a. H antigen
b. O antigen
c. K antigen
d. F antigen
ANS: a. H antigen - Gram+ organisms have ………………… pair of flagellar rings
a. 1
b. 2
c. 3
d. 4
ANS: a. 1 - Sheathed flagellum is seen in ……………………………….
a. Listeria
b. Leptospira
c. Salmonella
d. Leuconostoc
ANS: b. Leptospira - Which among the following shows pleomorphism?
a. Streptobacilli
b. Streptococci
c. Staphylococci
d. Lactobacilli
ANS: a. Streptobacilli - Quantitative ability of an organism to cause disease is ……………………………..
a. Virulence
b. Pathogenicity
c. Invasiveness
d. Adhesiveness
ANS: a. Virulence - ……………………………………. Test is used to distinguish pathogenic Staphylococci
a. Catalase test
b. Coagulase test
c. CAMP test
d. MUG test
ANS: b. Coagulase test - …………………………….. is the indicator used in Citrate utilization test
a. Bromocresol purple
b. Bromothymol blue
c. Neutral red
d. Phenol red
ANS: b. Bromothymol blue - Sodium thaurochorate is a ……………………..
a. Bile salt
b. Buffer
c. Bile pigment
d. PH indicator
ANS: a. Bile salt - ………………………………… pathway is used by Pseudomonas to oxidise aromatic compound to acetyl coA AND SUCCINATE
a. TCA cycle
b. Pyruvate decarboxylation pathway
c. Entner-Doudoroff pathway
d. Beta keto adipate pathway
ANS: d. Beta keto adipate pathway - Which among the following show bipolar staining characters?
a. Pasteurella and Salmonella
b. Pasteurella and Yersinia
c. Yersinia and Brucella
d. Yersinia and Salmonella
ANS: b. Pasteurella and Yersinia - Common laboratory contamionator is ………………………..
a. Pseudomonas aeruginosa
b. Protius vulgaris
c. Mycoplasma mycoides
d. Staphylococcus aureus
ANS: b. Protius vulgaris - Which among the following is true about Bacilllus anthracis?
a. Rough colony is pathogenic
b. Smooth colony is pathogenic
c. Mucoid colony is pathogenic
d. Both a & b
ANS: a. Rough colony is pathogenic - Type culture collections in India is maintained at …………………………
a. Ludhiana
b. Mukteshwar
b. Bareilly
c. Banglore
ANS: a. Ludhiana - ……………………………… is a type specimen designated by the original discoverer of the species
a. Neotype
b. Holotype
c. Serotype
d. Biotype
ANS: b. Holotype - ……………………………. Is a method of classification of bacteria using a number of parameters
a. Adansonian classification
b. Numerical classification
c. Polyphasic classification
d. Computer taxonomy
ANS: c. Polyphasic classification
Part 2:
- The ratio of diameter of objective lens to focal length is:
a. Numerical aperture
b. Resolution
c. Magnification
d. None of these - An example for positive eye piece:
a. Huygenian eyepiece
b. Ramsden eyepiece
c. Both a and b
d. None of these - ________________ microscopy is used to determine dry mass of living cells and their nuclei
a. Darkfield microscopy
b. Laser microscopy
c. Interference microscopy
d. Flourescence microscopy - ________________ types of specimens have to be used in electron microscopy
a. Hydrated specimen
b. Dehydrated specimen
c. Thick specimen
d. Stained specimen - Which among the following contain sterol in cytoplasmic membrane
a. Spirochetes
b. Mycoplasma
c. Clamydia
d. Clostridia - _________________ are also known as PPLO
a. Rickettsiae
b. Mycoplasma
c. Mycobacteria
d. Clamydia - ________________ produces packet of 8 organisms or multiple of 8.
a. Neisseria
b. Sarcina
c. Veillonella
d. All the above - Normal fir tree appearence in gelatin stab medium is produced by
a. Bacillus anthracis
b. Clostridium
c. Erysipelothrix
d. Salmonella - Primary stain in gram staining is
a. Crystal violet
b. Methylene blue
c. Safranin
d. Carbol fuchsin - In Hucker staining method, gram positive cell appear ________________ in colour
a. Blue
b. Pink
c. Violet
d. Dark red - Which among the following is wrong about negative staining of bacteria?
a. Used to study cell shape
b. Can be done with eosin or nigrosin
c. Stain does not penetrate the cell
d. Heat fixation is required - Which among the following is acid fast positive?
a. Mycoplasma
b. Nocardia
c. Neisseria
d. Fusobacterium - Traunt staining fluorescence method is used for
a. Endoscope
b. Acid fast bacteria
c. Flagella
d. Gram positive bacteria - Popping test is done for the staining of
a. Exospore
b. Endospore
c. Pili
d. Flagella - Which among the following is used for endospore staining
a. Schaeffer-Fulton staining
b. Donner staining method
c. Burke staining method
d. Both a and b - Which among the following is used for flagellar staining?
a. Gray staining method
b. Leyson staining method
c. Dorner staining method
d. Both a and b - ______________ is used in Ames test
a. Salmonella typhimurium
b. Shigella dysentriae
c. Staphylococcus aureus
d. Sreptococcus pneumoniae - Muller Hinton agar is used in
a. Ames test
b. Replica plating method
c. Antibiotic sensitivity test
d. Penicillin enrichment of mutants - Calcium chloride treated bacterial cells are competent for
a. Transduction
b. Transformation
c. Plasmid replication
d. Conjugation - Indicator in urea broth medium is _____________________
a. Phenol red
b. Bromocresol purple
c. Methyl red
d. Bromothymol blue - IMViC reaction for Yersinia is
a. + + – –
b. – + – +
c. – – + +
d. – + – – - Indole production can be detected by _____________________
a. Barritt’s reagent
b. Kovac’s reagent
c. Collin’s reagent
d. Baeyer’s reagent - Which among the following shows mixed acid fermentation of glucose
a. Shigella
b. Klebsiella
c. Enterobacter
d. Serratia - Which among the following is used as cryoprotectants in the storage of bacterial culture at -700C?
a. 10% glycerol
b. 10% DMSO
c. 15% glycerol
d. 15% DMSO - Voges proskeur test is based on the ability of organism to produce ________________ in glucose phosphate broth
a. Urea
b. Pyruvic acid
c. Acetoin
d. Phenylene diamine
ANSWERS: 1. a 2. b 3. c 4. b 5. b 6. b 7. b 8. b 9. a 10. c 11. d 12. b 13. b 14. b 15. d 16. d 17. a 18. c 19. b 20. a 21. d 22. b 23. a 24. a 25. c
Part 3:
- MIASMATIC THEORY was proposed by ……………………………………….
a. Themisson
b. Girolamo Frocaistoro
c. Thassalus
d. Hippocrates
Ans : d. Hippocrates - CONTAGION THEORY was proposed by ……………………………………….
a. Girolamo Frocaistro
b. Anton Von Leeuwenhoek
c. Athanisius Kircher
d. Francesco Redi
Ans : a. Girolamo Frocaistro - Louis Pasteur developed vaccines against the following diseases except …………………………
a. Anthrax
b. Fowl cholera
c. Tuberculosis
d. Rabies
Ans : Tuberculosis - The first person to use a solid medium for cultivating bacteria …………………………………………….
a. Louis Pasteur
b. Francesco Redi
c. Robert Koch
d. Theoder Schwann
Ans : c. Robert Koch - IVRI was established as Imperial Bacteriological laboratory in ……………………………………………………………
a. Pune ,1988
b. Muktheswar,1980
c. Pune, 1980
d. Muktheswar,1980
Ans: a. Pune,1988 - Organisms lacking a rigid cell wall are included under …………………………………………………………….
a. Gracilicutes
b. Firmicutes
c. Mendosicutes
d. Tenericutes
Ans: d. Tenericutes - Gram positive bacterial cellwall in addition to peptidoglycan contains …………………………………………..
a. Mycolic acid
b. Teichioc acid
c. Muramic acid
d. Pimilic acid
Ans : b. Teichoic acid - Limkage between N-acetyl glycosamine and N- cetyl muramic acid is …………………………………………….
a. beta 1,4
b. beta 1,3
c. beta 1,2
d. beta 1,5
Ans : a. beta 1,4 - Capsule and Slime layer are together known as …………………………………………
a. Endoplasmin
b. Glycocalyx
c. Exotoxin
d. Somatic antigen
Ans : b. Glycocalyx - Based on the arrangement of flagella Pseudomaonas aeuruginosa is …………………………………….
a. Peritrichous
b. Amphitrichous
c. Lophotrichous
d. Monotrichous
Ans: c. Lophotrichous - Which among the cocci organism have/has flagella?
a. Sporocercina
b. Rhodococcus
c. Planococcus
d. All the above
Ans: d. all the above - Impermeability of spore is due to ……………………………………………….
a. Poly-D-Glutamic acid
b. Calcium Dipicolinic acid
c. Thecoic acid
d. Diamino Pimilic acid
Ans: b. Calcium Dipicolinic acid - Which among the following is a diplococci?
a. Micrococci
b. Neisseria
c. Streptococci
d. Rhodococci
Ans: b. Neisseria - Feulgen staining is used to stain …………………………… which appears ………………………….. in colour.
a. Nucleoid, purple
b. Cytoplasm, blue
c. Ribosome, purple
d. volutin granules, red
Ans : a. Nucleoid, purple - Cooked meat media can be used for the culture of …………………………………..
a. Anaerobic
b. Capnophilic
c. Mesophilic
d. Thermophilic
Ans: a. Anaerobic - The method used to preserve bacteria in powder form is …………………………………..
a. Tyndallisation
b. Lyophilization
c. Deep freezing
d. Cryopreservation
Ans: b. Lyophilization - Refractive index of Canada Balsam is ………………………………..
a. 1.535
b. 1.524
c. 1.483
d. 1.460
Ans: a. 1.535 - ……………………………………………………… are produced by gram positive bacteria where there is complete absence of cell wall.
a. L-form
b. Spheroplast
c. Protoplast
d. Mycoplasma
Ans: c. Protoplast - Fractional sterilization was devised by ………………………………………….
a. Louis Pasteur
b. John Tyndall
c. John Snow
d. Joseph Lister
Ans: b. John Tyndall - Seitz Filter is used for filtering ………………………………………
a. Culture broth
b. Serum
c. Toxin from culture
d. Viral growth medium
Ans: b. Serum - Preservative used for serum is ………………………………..
a. Merthiolate
b. HCHO
c. 70% alcohol
d. 10% alcohol
Ans: a. Merthiolate - Garrod test is used ………………………………….
a. to evaluate virulence of bacteria
b. to check phenol coefficient
c. to check antibiotic susceptibility
d. to estimate D-value
Ans: b. to check phenol coefficient - Anaerobes cannot withstand oxygen due to lack of ………………………………….
a. Superoxide dismutase
b. Catalase
c. Peroxidase
d. both a and b
Ans: d. both a and b - Piezophiles are bacteria which can withstand great ……………………………….
a. atmospheric pressure
b. osmotic pressure
c. radiation
d. temperature
Ans: a. atmospheric pressure - Carbohydrates are added to the bacterial media at a rate of …………………………….
a. 0.5 – 2 %
b. 4 – 5 %
c. not added
d. 7%
Ans: a. 0.5 – 2 %
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